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CHEN HAI-BAO, Professor
Department of Bio-organic Chemistry
Date of Birth: 1938. 2
Education:
Graduated from Chemistry Department of Perkin University (1962)
from postgraduate study (tutor: Prof. Wang, Yu) in SIOC (1966)
As a visiting scholar in Khorana's Lab of M.I.T., USA (1984 - 1986)
Jion in SIOC: 1966
Tel: 86-21-64163300(ext.1429 or 1531)
E-mail: [email protected]; or E-mail: [email protected]

Research Interests:
Total Synthesize Derivatives of Dihydrostreptosonic monoacid g-lactone (1966)
Studies on Preparing Reversible Absorbent of CO2 for Nuclear Submarine (1968)
Nucleic Acids Chemistry:
Total Synthesis of Yeast Alanine tRNA (1970-1982)
Genetic Engineering and Protein Engineering:
Protein Engineering on Bovine Rhodopsin (1984-1986)
Genetic Engineering on Trichosanthin (1988-1994)
Genetic Engineering and Protein Engineering on higher Plant RubisCO (1988-)
Metabolic Engineering on CO2 Fixation (1995-)
Chemical Biology:
Screening the target protein with encoding gene of active organic compound (2000-)
Achivement Award:
First Class National Natural Science Prize (1987)
Selected Publication:
1. Total synthesis of yeast alanine tRNA, Scientia Sinica [ B series] (1983)
2. Cysteine residue 110 and 187 are essential for the formation of correct
structure in bovine rhodopsin, Proc. Natl. Acad. Sci. USA.(1988)
3. A new method for the synthesis of a structural gene, Nucl. Acids Res. (1990).
4. Design, total synthesis and highly expression in E.coli of a structural
gene for trichosanthin, Progress in Natural Science (in Chinese, 1995)
5. Studies on genetic engineering of rice Rubisco, Photosynthesis: from
light to biosphere (1995).
6. Overexpression in Escherichia coli and Characterization of the
Chloroplast Triosephosphate Isomerase from Spinach. Protein Expression
and Purification (1999)
7. Properties of Hybrid Enzymes between Synechococcus Large Subunits and Higher Plant Small Subunits of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Escherichia coli, Archives of Biochemistry and Biophysics (2001)
8. Two-cistron system overexpression of chloroplast glyceraldehyde-3-phosphate dehydrogenase subunit B and B-derived derivatives from spinach in Escherichia coli, Protein Expression and Purification (2001)
9. Co-expression of triosephophate isomerase, fructose-1,6-bis-phosphate aldolase and fructose-1,6-bisphosphatase in E. coli, Acta Biochimica et Biophysica Sinica (2001) (in Chinese).

Publications:

1. Huang Ying and Chen, H.-B., Active Recombinant Alpha Scorpion Neurotoxin rBmKaIT1--Gene Synthesis, Expression in Escherichia coli, Purification and Characterization, in the Proceedings of 7th Chinese (international) peptide symposium July, 2002 Dalian, China. To be published.
2. Wang, Y.-L., Zhou, J.-H., Wang, Y.-F., Bao, J.-Sh., and Chen, H.-B., Properties of Hybrid Enzymes between Synechococcus Large Subunits and Higher Plant Small Subunits of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Escherichia coli, Archives of Biochemistry and Biophysics (2001) 396, 35-42.
3. Jiang, H., Ma, J.-B., Zhu, X.-M., Hui, Y.-Zh., Wu, H.-M. and Chen, H.-B., Synthesis of biotinylated cyclosporin A and studies on its interaction with human cyclophilin A, Acta Chimica Sinica (2001) 59(10), 1745-1750 (in Chinese).
4. Tang, G.-L., Wang, Y.-F., Bao, J.-Sh. and Chen，H.-B., Two-cistron system overexpression of chloroplast glyceraldehyde-3-phosphate dehydrogenase subunit B and B-derived derivatives from spinach in Escherichia coli, Protein Expression and Purification (2001), 22(1), 31-37.
5. Tang, G.-L., Yang, Ch.-S., Wang, Y.-F., Bao, J.-Sh., Chen，H.-B., Shi, D.-J. & Liu, F.-L., Co-expression of triosephophate isomerase, fructose-1,6-bisphosphate aldolase and fructose-1,6-bisphosphatase in E. coli, Acta Biochimica et Biophysica Sinica (2001), 33 (1), 131-136 (in Chinese).
生物化学与生物物理学报（2001），33(1), 131-136。
6. Tang, G.-L., Ma, J.-B., Wu, H.-M. & Chen，H.-B., Novel technologies for study on the interaction between active small molecules and biological targets，Kexue Tongbao (2000), 45, 1345-1357 (in Chinese).
科学通报（2000），45，1345～1357。
7. Tang, G.-L. & Chen，H.-B., Recent development in metabolic engineering，YouJi HuaXue (2000) 20, 634-640 (in Chinese)
有机化学（2000），20，634－640。
8. 陈海宝聂慧玲，天花粉蛋白结构基因的DNA顺序测定，《天花粉蛋白》第二版，汪猷金善炜编，科学出版社，北京，pp.211～217，2000年6月.
9. 陈海宝聂慧玲，基因工程，《天花粉蛋白》第二版，汪猷金善炜编，科学出版社，北京，pp.218～224，2000年6月.
10. Tang, G.-L., Wang, Y.-F., Bao, J.-Sh. and Chen，H.-B., Overexpression in Escherichia coli and Characterization of the Chloroplast Fructose-1, 6- bisphosphatase from Wheat, Protein Expression and Purification (2000) 19, 411-418.
11. Zhou, J.-H. & Chen, H.-B., A facile DNA shuffling protocol, progress in Biochemistry & Biophysics (2000)，27, 655-657（in Chinese）。
周佳海，陈海宝，生物化学与生物物理进展 (2000)，27, 655-657.
12. Wu, Y.-L., Chen, H.-B., Jiang, K., Li Y., Shan, F., Wang, D.-Y., Wang, Y.-F., Wu, W-M., Wu, Y.-K., Yao, Z.-J., Yue, Z.-Y. And Zhou, C.-M., Interaction of biomolecules with qinghaosu(artemisinin) and its derivatives in the presence od ferrous ion ? an exploration of antimalarial mechanism, Pure Appl. Chem. (1999) 71, 1139-1142.
13. Tang, G.-L., Wang, Y.-F., Bao, J.-Sh. and Chen，H.-B., Overexpression in Escherichia coli and Characterization of the Chloroplast Triosephosphate Isomerase from Spinach, Protein Expression and Purification (1999) 16, 432－439.
14. Chen, H.-B .& Yang Ch.-S., Cloning and high expression in E.coli of a chimeric gene coding for rice fructose-1,6-bisphosphate aldolase, Acta Biochimica et Biophysica Sinica, (1997) 29, 613-616 (in Chinese).
生物化学与生物物理学报 (1997)29, 613-616.
15. Wu, W.-M., Yao, Z.-J., Wu, Y.-L., Jiang, K., Wang, Y.-F., Chen, H.-B., Shan, F., Li, Y., Ferrous Ion Induced Cleavage of the Peroxy Bond in Qinghaosu and its Derivatives and the DNA damage Associated with this Process, Chem. Commun. (1996), 2213-2214.
16. Wang, G.-A., Chen, H.-B., Wang, Y., Studies on genetic engineering of rice Rubisco, in "Photosynthesis: from lightto biosphere" Vol V,, Mathis, P. Ed., Kluwer Academic Publishers, Netherlands, pp. 91-94, 1995.
17. 陈海宝夏懿荆军平蒋昆鲍建绍,天花粉蛋白结构基因的重新设计、全合成及其在大肠杆菌中的高表达,自然科学进展- 国家重点实验室通讯(1995）第5卷第1期, 114－118页。.
18. Chen, H.-B., Xia, Y., Jing, J.-P., Jiang, K., Bao, J.-Sh., Chemical Synthesis of a Structural Gene Coding for Trichosanthin, Chinese J. Chem. (1995) 13:4, 349-357.
19. Chen, H.-B., Weng, J.-M., Bao, J.-Sh., Chemical Synthesis of a Structural Gene Coding for Small Subunit of Ribulose-1,5-bisphosphate caboxylase / oxygenase from Tobacco, Chinese J. Chem. (1994) 12, 355-364.
20. 陈海宝，1993年诺贝尔化学奖简介 ? 简评化学研究在生物学科发展中的贡献和展望，HuaXue TongBao (1994) 8: 38-40.
21. 陈海宝，开创研究遗传物质的新方法，Science pictorial (1994) No.1, 8-9.
22. Chen, H.-B., Wang, G.-A., Synthesis and Expression of a Gene for Mature Small subunit of Rice Rubisco, Curr. Plant Sci. Biotecnol. Agric. (1993) 15 (Biotechnology in Agriculture), 160-164.
23. Chen, H.-B., Total Synthesis and Expression in E. coli of Three Redesigned Genes for the Mature Small Subunit ( rbcS ) of Rubisco from Tobacco, Wheat and Rice, YouJi HuaXue ( Chinese J. of Organic Chemistry) (1993) 13, 309-313.
24. Chen, H.-B., Li, J.-W., Yang, J., Expression in E. coli of a Chemically Synthesized Gene for the Mature rbcS from Tobacco, in "Research in Photosynthesis", Vol. III, Ed. by Murata, N., Kluwer Academic Publishers, Netherlands, pp. 645-648, 1992.
25. 陈海宝刘国诠，化学与未来的生物学，王亚辉吴志纯主编的"走向21世纪的生物学 ? 未来生物学(1991－2020)年预测" 中国科学院科技政策局，华夏出版社出版， pp168-182, 1992.
26. Chen, H.-B., Weng, J.-M., Bao, J.-Sh., Total Synthesis of a Gene for the Mature Small Subnuit of RubisCO from Tobacco, in "Current Research on Photosynthesis", Vol. III, Ed. by Baltscheffsky, M., Kluwer Academic Publishers, Netherlands, pp. 347-351, 1990.
27. Chen, H.-B., Weng, J.-M., Jiang, K., Bao, J.-Sh., A New Method for the Synthesis of a Structural Gene, Nucleic Acids Research (1990) 18, 871-878.
28. 陈海宝，核酸的有机化学研究在分子生物学发展中的作用，YouJi HuaXue (1990) 10, 298-327.
29. Braiman, M., Bubis, J., Chen, H.-B., Doi, T., Flitsch, S.L., Franke, R.R., Gilles-Gonzalez, M.A., Graham, R.M., Karnik, S.S., Khorana, H.G., Knox, B.E., Krebs, M.P., Marti, T., Mogi, T., Nakayama, T., Oprian, D.D., Puckett, K.L., Sakmar, T.P., Stern, L.J., Subramaniam, S. & Thompson, D.A. Studies on Light Transduction by Bacteriorhodopsin and Rhodopsin, Cold Spring Harbor Symp. Quant. Biol. (1988) 53, 355-364.
30. Karnik, S.S., Sakmar, T.P., Chen, H.-B., Khorana, H.G., Cysteines 110 and 187 are Essential for the Formatiion of Correct Structure in Bovine Rhodopsin, Proc. Natl. Acad. Sci. USA (1988) 85, 8459-8463.
31. Karnik, S.S., Sakmar, T.P., Franke, R.R., Knox, B.E., Oprian, D.D., Chen, H.-B., Khorana, H. G. Site-specific Mutagenesis of Bovine Rhodopsin, in "Molecular Biology of the Eye: Genes, Vision and Ocular Disease", UCLA Symposium on Molecular and Cellular Biology, New Series, Vol. 88, Ed. by Pialigorsky, J., Shinohara, T. , and Zelenka, P. , Alan R. Liss, Inc., New York, NY., pp. , 23-44,1988.
32. Oprian, D.D., Sakmar, T.P., Nakayama, T., Chen, H.-B., Knox, B.E., Karnik, S.S., Franke, R. R., and Khorana, H. G. Molecule Biological Studies of Visual Pigment, Rhodopsin, in Discussions in Neurosciences FESN " Vol 4 (3), Ed. by Hudspeth, A.J., MacLeish, P.R., Margolis, F.L &Wiesel, T.N.,Geneva, Switzerland, pp. 20-29, 1987.
33. Chen, H.-B., Zhuang, J.-H., Zheng, Y.-D.& Hu, M.-H., Synthesis of the 5'-Half Molecule Pentatricontanucleotide (35p) of Yeast Alanine Transfer Ribonucleic Acid, Kexue Tongbao, (1984) 29, 109-115.
34. Chen, H.-B., Xu, J.-F., Bao, J.-Sh., Zhuang, J.-H., Guo, R.-H., Shi, C.-N.,Chen, F.-X., Zhang, G.-J., Fong, X.-L., Jiang, P., Ding, X.-H.,Synthesis of the 3'-Termina Nonadecanucleotide (58-76) of Yeas Alanine tRNA, Acta Chimica Sinica, Engl. Ed., (1983) 1, 212-229.
35. Wang, D.-B., Zheng, K.-Q., Qiu, M.-S., Liang, Z.-H., Wu, R.-L., Chen, C.-Q.,Wang, E.-B., Zhu, Y.-S., Shen, Q.-X., Yu, Y.-H., Wang, Y., Chen, H.-B., Yang, Z.-W., Lu, Y.-H., Chen, Z., Wang, G.-H., & Hu, M.-H., Total Synthesis of Yeast Alanine Transfer Ribonucleic Acid, Scientia sinica (series B，1983) 26, 464-481.
36. Wang, D.-B., Qiu, M.-S., Liang, Z.-H., Zheng, K.-Q., Wu, R.-L., Wang, C.-Y., Liu, X.-Y., Zheng, H.-D., Bao, Y.-D., Zhu, X.-L., Guo, L.-H., Chjen, Y.-D., Huang, J.-J., Zhou, F.-Y., Chen, H.-B., Xu, B.-Zh., Zhang, Q.-J., Hua, L., & Hu, M.-H., Synthesis of 3'-Half Molecule of Yeast Alanine tRNA, Scientia sinica (series B，1983) 26, 482-494.
37. Wang, D.-B., Wu, R.-L., Zheng, K.-Q., Yu, D.-W., Tang, J.-Y., Qi, G.-R., Chen, C.-Q., Zhu, Y.-S., Xie, H.-Y., Wang, Y.-K., Nie, H.-L., Chen, H.-B., Huang, J.-H., Chen, D.-H., Zhuang, J.-H., Wang, G.-H., Chen, S., & Hu, M.-H., Synthesis of 5'-Half Molecule of Yeast Alanine tRNA, Scientia sinica (series B，1983) 26, 495-503.
38. Shanghai Institute of Biochemistry, Academia Sinica; Shanghai Institute of Cell Biology, Academia Sinica; Shanghai Institute of Organic Chemistry, Academia Sinica; Institute of Biophysics, Academia Sinica; Department of Biology, Peking University and Shanghai No.2 Reagent Factory, Total Synthesis of Yeast Alanine Transfer Ribonucleic Acid, Kexue Tongbao(1982) 27, 261-269.
39. Collaboration Group of Nucleic Acid Synthesis, Studies on the Synthesis of Polynucleotides V. ---Synthesis of a Nonadecaribonucleotide Fragment (58-76) of the Yeast Alanine tRNA in "Nucleic Acids and Proteins", The 1979 Shanghai Proceedings of PRC-FRG Join Symposium on Nucleic Acids and Proteins, Ed. by Z.-W. Shen, pp. 272-281, Science Press, Beijing (van Nostrand Reinhold Company, New York/Toronto/London), 1980.
40. Collaboration Group for Nucleic Acid Synthesis, Synthesis of 3'-Half Molecule of Yeast Alanine tRNA, Kexue Tongbao, (1980) 25, 525-527.
41. Nucleic Acid Group, Shanghai Institute of Organic Chemistry, Academia Sinica, Studies on Nucleic Acid Chemistry III. Synthesis of ApUpUpC, CpGpGpA and ApUpUpCp of TUC Arm of Yeast Alanine tRNA, Acta Chimica Sinica (1980) 38, 1-17(in Chinese).
42. Nucleic Acid Group, Shanghai Institute of Organic Chemistry, Academia Sinica, Studies on Nucleic Acid Chemistry I. Crystalline 5'-O-Trityl and 5'-O-Mono-p-Methoxytrityl Ribonucleotides, Acta Chimica Sinica (1979) 37, 299-304 (in Chinese).
43. Nucleic Acid Group, Shanghai Institute of Organic Chemistry, Academia Sinica, Studies on Nucleic Acid Chemistry II. The Application of N-Acylimidazoles in the Acyla- tions of the Ribonucleotides, Acta Chimica Sinica (1979) 37, 305-314(in Chinese).
44. Collaboration Group of Nucleic Acid Synthesis, Studies on the Synthesis of Polynucleotides --- Synthesis of a Dodecaribonucleoside Undeca-phosphate and Hexadecaribonucleoside Pentadecaphosphate, Scientia Sinica, (1978) 21, 687-697.
45. 陈海宝，有机化学方法合成寡聚核糖核苷酸的概况，Youji Huaxue (1976) No 1: 47-60.