Ta-Yuan Chang

 

Mailing Address:

Department of Biochemistry

Dartmouth Medical School

Hanover, NH 03755

Tel. 603-650-1622

Fax. 603-650-1128

e-mail: [email protected]

Chang Laboratory Website: http://www.Dartmouth.edu/~tychang

 

Date of Birth: April 8, 1945

 

Education:

B.S. in Chemistry, National Taiwan University, Taiwan, 1967

Ph.D. in Biochemistry (with Mary Ellen Jones), University of North Carolina, Chapel Hill, North Carolina, 1968-1973

Postdoctoral Fellow (with Roy Vagelos), Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri, 1973-1975

Postdoctoral Fellow (with Roy Vagelos), Merck, Sharp & Dohme Research Laboratories, Rahway, New Jersey, 1975-1976

 

Professional Experience:

2000-present Professor and Chair, Department of Biochemistry, Dartmouth Medical School

1988-2000 Professor, Department of Biochemistry,
Dartmouth Medical School

1982-1988 Associate Professor, Department of Biochemistry, Dartmouth Medical School

1976-1982 Assistant Professor, Department of Biochemistry, Dartmouth Medical School

 

Membership:

American Society of Biological Chemists and Molecular Biologists, 1981-present

American Society of Cell Biologists, 1999-present

 

Extramural Activities:

Member of Editorial Board, Journal of Biological Chemistry (1995-2000)

Member of Editorial Board, Journal of Biological Chemistry (2001-2006)

Visiting Professor, Kumamoto University Medical School, Kumamoto, Japan (1995-1997; 1999-2000)

Member, American Heart Association National Review Committee on Lipids and Lipoproteins (1998-2002)

Visiting Professor, University of Hong Kong (2003)

 

Honors:

NIH Research Career Development Award (1982-1987)

NIH MERIT Award (1994-2004)

 

Current Research Support:

1. NIH R37 HL36709 (1994-2004) Cell Mutants Defective in Cholesterol Ester Formation

2. NIH RO1 HL60306 (2003-2007) Structure-Function Analyses of ACAT

 

Invited Speaker at Selected National and International Meetings, 1987-2003:

  1. American Society of Biological Chemists Satellite Symposium on Cellular Cholesterol Metabolism (1987), Chair: Dr. Michael Sinensky
  2. Aspen Cholesterol Conference (1987), Chair: Dr. Roger Davis
  3. Gordon Research Conference on Atherosclerosis (1989), Chairs: Drs. Thomas Innerarity and Alan Fogelman
  4. International Symposium on Drugs Affecting Lipid Metabolism, Houston, Texas (1989), Chair: Dr. Antonio Gotto
  5. Aspen Cholesterol Conference (1991), Chairs: Drs. Daniel Steinberg and Donald Small
  6. Japanese Research Conference on Cholesterol, Shirahama, Japan (1991), Chair: Dr. Seikoh Horiuchi
  7. International Conference on Atherosclerosis and Thrombosis, Tokyo, Japan (1992), Chair: Dr. Akira Yamamoto
  8. Aspen Cholesterol Conference (1993), Chair: Dr. Larry Rudel
  9. Xth International Symposium on Atherosclerosis, Montreal, Canada (1994), Chair: Dr. Jean Davignon
  10. Aspen Cholesterol Conference (1995), Chair: Dr. John Dietschy
  11. American Heart Association National Meeting (1996), State of the Art Speaker in Lipid Metabolism Session
  12. FASEB Summer Research Conference on Intestinal Lipid Digestion (1997), Chair: Dr. David Hui
  13. International Satellite Conference on Cellular Cholesterol Metabolism, San Francisco, California (1997), Chair: Dr. Sandra Erickson
  14. Gordon Research Conference on Lipid Metabolism (1998), Chair: Dr. David Williams
  15. International Symposium on Lipoprotein Metabolism and Atherogenesis, Kyoto, Japan (1998), Chair: Dr. Toru Kita
  16. International Conference on the NPC Lesion and the Role of Intracellular Cholesterol Trafficking in Human Disease, Washington DC (1999), Chair: Dr. Peter Pentchev
  17. FASEB Summer Research Conference on Intestinal Lipid Digestion (2000), Chair: Dr. Jeff Field
  18. ZMBH Forum (2001), "Genes, Proteins, and Brain Disease", Heidelberg, Germany. Organizer: Tobias Hartmann
  19. Gordon Research Conference on Molecular and Cell Biology of Lipids (2001), Chair: Charles Rock
  20. Gordon Research Conference on Lipoprotein Metabolism (2002), Session Chair and Speaker, Chair: Mike Phillips.
  21. American Heart Association National Meeting (2002), Session Chair and Speaker

 

Publications in Peer-Reviewed Journals:

  1. Chang, T.Y. and Jones, M.E. Aspartate Transcarbamylase from Streptococcus faecalis. Purification, Properties and Nature of an Allosteric Activator Site. Biochemistry 13, 629 (1974)
  2. Chang, T.Y. and Jones, M.E. Aspartate Transcarbamylase from Streptococcus faecalis. Steady-State Kinetic Analysis. Biochemistry 13, 637 (1974)
  3. Chang, T.Y. and Jones, M.E. Aspartate Transcarbamylase from Streptococcus faecalis. Reverse Reaction and Binding Studies. Biochemistry 13, 646 (1974)
  4. Chang, T.Y. and Vagelos, P.R. Isolation and Characterization of an Unsaturated Fatty Acid Requiring Mutant of Cultured Mammalian Cells. Proc. Natl. Acad. Sci. U.S.A. 73, 24 (1976)
  5. Chang, T.Y., Telakowski, C., Vanden Heuvel, W., Alberts, A.W., and Vagelos, P.R. Isolation and Partial Characterization of a Cholesterol-Requiring Mutant of Chinese Hamster Ovary Cells. Proc. Natl. Acad. Sci. U.S.A. 74, 833 (1977)
  6. Nelson, J.A., Czarny, M.R., Spencer, T.A., Limanek, J.S., McCrae, K.R., and Chang, T.Y. A Novel Inhibitor of Steroid Biosynthesis. J. Am. Chem. Soc. 100, 4900 (1978)
  7. Limanek, J.S., Chin, J., and Chang, T.Y. A Mammalian Cell Mutant Requiring Cholesterol and Unsaturated Fatty Acid for Growth. Proc. Natl. Acad. Sci. U.S.A. 75, 5452 (1978)
  8. Chang, T.Y., Schiavoni, E.S., Jr., McCrae, K.R., Nelson, J.A., and Spencer, T.A. Inhibition of Cholesterol Biosynthesis in Chinese Hamster Ovary Cells by 4,4,10-Trimethyltransdecal-3b -o1. A Specific 2,3-Oxidosqualene Cyclase Inhibitor. J. Biol. Chem. 254, 11258 (1979)
  9. Chang, T.Y. and Limanek, J.S. Regulation of Cytosolic Acetoacetyl CoA Thiolase, 3-Hydroxy-3-Methylglutaryl CoA Synthase, 3-Hydroxy-3-Methylglutaryl CoA Reductase, and Mevalonate Kinase by Low Density Lipoprotein and by 25-Hydroxycholesterol in Chinese Hamster Ovary Cells. J. Biol. Chem. 255, 7787 (1980)
  10. Chang, T.Y., Limanek, J.S., and Chang, C.C.Y. Evidence Indicating that Inactivation of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase by Low Density Lipoprotein or by 25-Hydroxycholesterol Requires Mediator Protein(s) with Rapid Turnover Rate. J. Biol. Chem. 256, 6174 (1981)
  11. Chin, J. and Chang, T.Y. Evidence for Coordinate Expression of 3-Hydroxy-3-Methylglutaryl Coenzyme A. Reductase and Low Density Lipoprotein Binding Activity. J. Biol. Chem. 256, 6304 (1981)
  12. Chang, T.Y., Limanek, J.S., and Chang, C.C.Y. A Simple and Efficient Procedure for the Rapid Homogenization of Cultured Animal Cells Grown in Monolayer. Anal. Biochem. 116, 298 (1981)
  13. Berry, D.J. and Chang, T.Y. Further Characterization of a Chinese Hamster Ovary Cell Mutant Defective in Lanosterol Demethylation. Biochemistry 21, 573 (1982)
  14. Doolittle, G.M. and Chang, T.Y. Solubilization, Partial Purification and Reconstitution in Phosphatidylcholine-Cholesterol Liposomes of Acyl-CoA:Cholesterol Acyltransferase. Biochemistry 21, 674 (1982)
  15. Chin, J. and Chang, T.Y. Further Characterization of a Chinese Hamster Ovary Cell Mutant Requiring Cholesterol and Unsaturated Fatty Acid for Growth. Biochemistry 21, 3196 (1982)
  16. Chang, T.Y. and Chang, C.C.Y. Revertants of Chinese Hamster Ovary Cell Mutant Resistant to Suppression by Analog of Cholesterol-Isolation and Partial Characterization. Biochemistry 21, 5316 (1982)
  17. Doolittle, G.M. and Chang, T.Y. Acyl-CoA:Cholesterol Acyltransferase in Chinese Hamster Ovary Cells: Enzyme Activity Determined after Reconstitution in Phospholipid-Cholesterol Liposomes. Biochim. Biophys. Acta 713, 529 (1982)
  18. Chang, C.C.Y., Doolittle, G.M., and Chang, T.Y. Cycloheximide-Sensitivity in Regulation of Acyl-CoA:Cholesterol Acyltransferase in Chinese Hamster Ovary Cells. I. Effect of Exogenous Sterols. Biochemistry 25, 1693 (1986)
  19. Chang, C.C.Y. and Chang, T.Y. Cycloheximide-Sensitivity in Regulation of Acyl-CoA:Cholesterol Acyltransferase in Chinese Hamster Ovary Cells. II. Effect of Endogenous Sterol Synthesis. Biochemistry 25, 1700 (1986)
  20. Ventimiglia, J., Levesque, M.C, and Chang, T.Y. Preparation and Characterization of Unilamellar Vesicles from Cholate-Phospholipid Micelle Treated with Cholestyramine. Anal. Biochem. 157, 323 (1986)
  21. Cadigan, K., Heider, J., and Chang, T.Y. Isolation and Characterization of Chinese Hamster Ovary Cell Mutants Deficient in Acyl-Coenzyme A:Cholesterol Acyltransferase Activity. J. Biol. Chem. 263, 274 (1988)
  22. Cadigan, K.M. and Chang, T.Y. A Simple Method for Reconstituting Acyl-CoA:Cholesterol Acyltransferase in Cholesterol Containing Liposome. J. Lipid Research 29, 1683 (1988)
  23. Cadigan, K.M., Chang, C.C.Y., and Chang, T.Y. Isolation of Chinese Hamster Ovary Cell Lines Expressing Human Acyl- Coenzyme A:Cholesterol Acyltransferase Activity. J. Cell Biol. 108, 2201 (1989)
  24. Shi, S.P., Chang, C.C.Y., Gould, G.W., and Chang, T.Y. Comparing the Unilamellar Phosphatidylethanolamine Vesicles with the Unilamellar Phosphatidylcholine Vesicles Produced by Treating Cholate-phospholipid Micelle with Cholestyramine. Biochim. Biophys. Acta 982, 187 (1989)
  25. Cadigan, K.M., Spillane, D.M., and Chang, T.Y. Isolation and Characterization of Chinese Hamster Ovary Cell Mutants Defective in Intracellular Low Density Lipoprotein-Cholesterol Trafficking. J. Cell Biol. 110, 295 (1990)
  26. Hasan, M.T., Subbaroyan, R., and Chang, T.Y. High-efficiency Stable Gene Transfection using Chloroquine-treated CHO cells. Somatic Cell & Mol. Genetics 17, 513 (1991)
  27. Chang, C.C.Y., Huh, H.Y., Cadigan, K.M., and Chang, T.Y. Molecular Cloning and Functional Expression of Human Acyl- CoA:Cholesterol Acyltransferase cDNA in Mutant Chinese Hamster Ovary Cells. J. Biol. Chem. 268, 20747 (1993)
  28. Hasan, M.T., Chang, C.C.Y., and Chang, T.Y. Somatic Cell Genetic and Biochemical Characterization of Cell Lines Resulting from Human Genomic DNA Transfection of Chinese Hamster Ovary Cell Mutants Defective in Sterol-Dependent Activation of Sterol Synthesis and LDL Receptor Expression. Somatic Cell & Mol. Genetics 20, 183 (1994)
  29. Chang, C.C.Y., Nutile-McMenemy, N., Noll, W., Lindsay, E.A., Baldini, A., Chang, W., and Chang, T.Y. Localization of Acyl-Coenzyme A:Cholesterol Acyltransferase Gene to Human Chromosome 1q-25. Somatic Cell & Mol. Genetics 20, 71 (1994)
  30. Hasan, M.T. and Chang, T.Y. Somatic Cell Genetic Analysis of Two Classes of CHO Cell Mutants Expressing Opposite Phenotypes in Sterol-Dependent Regulation of Cholesterol Metabolism. Somatic Cell & Mol. Genetics 20, 481 (1994)
  31. Spillane, D.M., Reagan, J.W., Kennedy, N.J., Schneider, D.L., and Chang, T.Y. Translocation of Both Lysosomal LDL-derived Cholesterol and Plasma Membrane Cholesterol to the Endoplasmic Reticulum for Esterification May Require Common Cellular Factors Present in the Acidic Compartment(s). Biochim. Biophys. Acta 1254, 283 (1995)
  32. Cheng, D., Chang, C.C.Y., Qu, X-M., and Chang, T.Y. Activation of Acyl-Coenzyme A:Cholesterol Acyltransferase (ACAT) by Cholesterol or by Oxysterol in a Cell-Free System. J. Biol. Chem. 270, 685 (1995)
  33. Uelmen, P.J., Oka, K., Sullivan, M., Chang, C.C.Y., Chang, T.Y., and Chan, L. Tissue-Specific Expression and Cholesterol Regulation of Acyl-Coenzyme A:Cholesterol Acyltransferase (ACAT) in Mice: Molecular Cloning of Mouse ACAT cDNA, Chromosomal Localization, and Regulation of ACAT In Vivo and In Vitro. J. Biol. Chem. 270, 26192 (1995)
  34. Chang, C.C.Y., Chen, J., Thomas, M., Cheng, D., Del Priore, V.A., Newton, R.S., Pape, M.E., and Chang, T.Y. Regulation and Immunolocalization of Acyl-Coenzyme A:Cholesterol Acyltransferase in Mammalian Cells as Studied with Specific Antibodies. J. Biol. Chem. 270, 29532 (1995)
  35. Matsuda, H., Hakamata, H., Miyazaki, A., Sakai, M., Chang, C.C.Y., Chang, T.Y., Kobori, S., Shichiri, M., and Horiuchi, S. Activation of Acyl-Coenzyme A:Cholesterol Acyltransferase Activity by Cholesterol is Not Due to Altered mRNA Levels in HepG2 Cells. Biochim. Biophys. Acta 1301, 76 (1996)
  36. Carstea, E.D., Morris, J.A., Coleman, K.G., Loftus, S.K., Zhang, D., Cummings, C., Gu, J., Rosenfeld, M.A., Pavan, W.J., Krizman, D.B., Nagle, J., Polymeropoulos, M.H., Sturley, S.L., Ioannou, Y.A., Higgins, M.E., Comly, M., Cooney, A., Brown, A., Kaneski, C.R., Blanchette-Mackie, E.J., Dwyer, N.K., Neufeld, E.B., Chang, T.Y., Liscum, L., Strauss, J.F., III, Ohno, K., Zeigler, M., Carmi, R., Sokol, J., Markie, D., O'Neill, R.R., Van Diggelen, O.P., Elleder, M., Patterson, M.C., Brady, R.O., Vanier, M.T., Pentchev, P.G., and Tagle, D.A. The Niemann-Pick C Gene: Homology to Mediators of Cholesterol Homeostasis. Science 277, 228 (1997)
  37. Rawson, R.B., Zelenski, N.G., Nijhawan, D., Ye, J., Sakai, J., Hasan, M.T., Chang, T.Y., Brown, M.S., and Goldstein, J.L. Complementation Cloning of S2P, a Gene Encoding a Putative Metalloprotease Required for Intramembrane Cleavage of SREBPs. Molecular Cell 1, 47 (1997)
  38. Khelef, N., Buton, X., Beatini, N., Wang, H., Meiner, V., Chang, T.Y., Farese, R.V. Jr, Maxfield, F.R., and Tabas, I. Immunolocalization of Acyl-Coenzyme A:Cholesterol Acyltransferase in Macrophages. J. Biol. Chem. 273, 11218 (1998)
  39. Li, O., Chang, C., Lee, W., and Chang, T.Y. Immunodepletion Experiments suggest that Acyl-Coenzyme A:Cholesterol Acyltransferase 1 Protein is the Major Catalytic Component in Adult Human Liver, Adrenal Gland, Kidney, and Macrophages, but not in Human Intestines. J. Lipid Res. 39, 1722 (1998)
  40. Miyazaki, A., Sakashita, N., Lee, O., Takahashi,S., Hakamata, H., Morganelli, P., Chang, C.C.Y., and Chang, T.Y. Expression of Acyl-Coenzyme A:Cholesterol Acyltransferase in Human Atherosclerotic Lesions and in Cultured Human Monocyte-Macrophages. Arterio. Thromb. and Vas. Biol. 18, 1568 (1998)
  41. Chang, C.C.Y., Lee, C.Y.G., Chang, E.T., Cruz, J.C., Levesque, M., and Chang, T.Y. Recombinant Acyl-CoA:Cholesterol Acyltransferase-1 (ACAT-1) Purified to Essential Homogeneity Utilizes Cholesterol in Mixed Micelles or in vesicles in a Highly Cooperative Manner. J. Biol. Chem. 273, 35132 (1998)
  42. Tomita, T., Chang, T.Y., Kodama, T., and Iwatsubo, T. ß-APP Gamma-Secretase and SREBP Site 2 Protease are two Different Enzymes. NeuroReport 9, 911 (1998)
  43. Li, B.L., Li, X.L., Duan, Z., Lee, O., Lin, S., Ma, Z.M., Chang, C.C.Y., Yang, X.Y., Mohandas, T.K., Noll, W., Chan, L., and Chang, T.Y. Human ACAT-1 Gene Organization, and the Evidence that the 4.3 kb mRNA is Produced from two Different Chromosomes. J. Biol. Chem. 274, 11060 (1999)
  44. Lin, S., Cheng, D., Liu, M.S., Chen, J., and Chang, T.Y. Human Acyl-CoA:Cholesterol Acyltransferase-1 (ACAT-1) in the Endoplasmic Reticulum Contains Seven Transmembrane Domains. J. Biol. Chem. 274, 23276 (1999)
  45. Yu, C., Chen, J., Lin, S., Liu, J., Chang, C.C.Y., and Chang, T.Y. Human Acyl-CoA:Cholesterol Acyltransferase-1 (ACAT-1) is a Homotetrameric Enzyme in Intact Cells and In Vitro. J. Biol. Chem. 274, 36139 (1999)
  46. Sakashita, N., Miyazaki, A., Takeya, M., Horiuchi, S., Chang, C.C.Y., Chang, T.Y., and Takahashi, K. Localization of Human Acyl-Coenzyme A:Cholesterol Acyltransferase-1 (ACAT-1) in Macrophages and in Various Tissues. Amer. J. Pathol. 156, 227 (2000)
  47. Cruz, J.C., Sugii, S., Yu, C., and Chang, T.Y. Role of Niemann-Pick type C1 protein in Intracellular Trafficking of Low Density Lipoprotein Derived Cholesterol. J. Biol. Chem. 275, 4013 (2000) (chosen as cover page)
  48. Henderson, L.P., Lin, L., Prasad, A., Paul, C.A., Chang, T.Y., and Maue, R.A. Embryonic Striatal Neurons from Niemann-Pick type C Mice Exhibit Defects in Cholesterol Metabolism and Growth Factor Responsiveness. J. Biol. Chem. 275, 20179 (2000) (chosen as Highlight of Recent Literature by Editor of Science, 289, Number 5477, 14 July 2000)
  49. Chang, C.C.Y., Sakashita, N., Ornvold, K., Lee, O., Chang, E., Dong, R., Lin, S., Lee, C.Y.G., Strom, S., Kashyap, R., Fung, J., Farese, R., Patoiseau, J.F., Delhon, A., and Chang, T.Y. Immunological Quantitation and Localization of ACAT-1 and ACAT-2 in Human Liver and Small Intestines. J. Biol. Chem. 275, 28083 (2000)
  50. Cruz, J.C., and Chang, T.Y. Fate of Endogenously Synthesized Cholesterol in Niemann-Pick Type C1 Cells. J. Biol. Chem. 275, 41309 (2000)
  51. Maung, K.K., Miyazaki, A., Nomiyama, H., Chang, C.C.Y., Chang, T.Y., and Horiuchi, S. Increase in acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1) mRNAs and protein contents by treating undifferentiated human monocytic THP-1 cells with 1,25-dihydroxyvitamin D3 or with 9-cis retinoic acid. J. Lipid Res. 42, 181 (2001)
  52. Yamazaki, T., Chang, T.Y., Haass, C., and Ihara, Y. Accumulation and aggregation of amyloid ß-protein in late endosomes of Niemann-Pick type C cells. J. Biol. Chem. 276, 4454 (2001)
  53. Yang, J.B., Duan, Z.J., Yao, W., Lee, O., Yang, L., Yang, X.Y., Sun, X., Chang, C.C.Y., Chang, T.Y., and Li, B.L. Synergistic Transcriptional Activation of Human ACAT-1 Gene by IFN-gamma and ATRA in THP-1 Cells. J. Biol. Chem. 276, 20989 (2001)
  54. Song, B.L., Qi, W., Yang, X.Y., Chang, C.C.Y., Zhu, J.Q., Chang, T.Y., and Li, B.L. Genomic Organization of Human ACAT-2 Gene and Cell Type- Specific Expressions of its Promoter. Biochem. Biophys. Res. Commun. 282, 580 (2001)
  55. Puglielli, L., Konopka, G., Pack-Chung, E., Ingano, L.A.M., Berezovska, O., Hyman, B.T., Chang, T.Y., Tanzi, R.E., and Kovacs, D.M. Acyl-coenzyme A:cholesterol acyltransferase modulates the generation of the amyloid beta-peptide. Nature Cell Biol. 3, 905 (2001)
  56. Lu, X.H., Lin, S., Chang, C.C.Y., Chang, T.Y. Mutant Acyl-coenzyme A:Cholesterol Acyltransferase 1 Devoid of Cysteine Residues Remains Catalytically Active. J. Biol. Chem. 277, 711 (2002)
  57. Yu, C., Zhang, Y., Lu, X., Chen, J., Chang, C.C.Y., and Chang, T.Y. Role of the N-Terminal Hydrophilic Domain of Acyl-Coenzyme A:Cholesterol Acyltransferase 1 on the Enzyme's Quaternary Structure and Catalytic Efficiency. Biochemistry 41, 3762 (2002)
  58. Cruz, J.C., Thomas, M., Wong, E., Ohgami, N., Sugii, S., Curphey, T., Chang, C.C.Y., and Chang, T.Y. Synthesis and Biochemical properties of a New Photoactivatable Cholesterol Analog 7,7-azocholestanol and its Linoleate Ester in Chinese Hamster Ovary Cell Lines. J. Lipid Res. 43, 1341 (2002)
  59. Buszczak, M., Lu, X., Segraves, W.A., Chang, T.Y., and Cooley, L. Mutations in the midway Gene Disrupt a Drosophila Acyl Coenzyme A: Diacylglycerol Acyltransferase. Genetics 160, 1511 (2002)
  60. Khan, N., Shen, J., Chang, T.Y., Chang, C.C.Y., Fung, P.C.W., Grinberg, O., Demidenko, E., and Swartz, H. Plasma Membrane Cholesterol: A Possible Barrier to Intracellular Oxygen in Normal and Mutant CHO Cells Defective in Cholesterol Metabolism. Biochemistry 42(1), 23 (2003)
  61. Zhang, Y., Yu, C., Liu, J., Spencer, T.A., Chang, C.C.Y., and Chang, T.Y. Cholesterol is superior to 7-ketocholesterol or 7alpha-hydroxycholesterol as an allosteric activator for acyl-coenzyme A: cholesterol acyltransferase 1. J. Biol. Chem. In press/Published online Jan. 17 (2003)
  62. Sugii, S., Reid, P.C., Ohgami, N., Shimada, Y., Maue, R.A., Ninomiya, H., Ohno-Iwashita, Y., and Chang, T.Y. Biotinylated theta toxin derivative as a probe to examine intracellular cholesterol-rich domains in normal and Niemann-pick type C1 cells. J. Lipid Res. In press/Published online Feb. 1 (2003)
  63. Reid, P.C., Sugii, S., and Chang, T.Y. Trafficking defects in endogenously synthesized cholesterol in fibroblasts, macrophages, hepatocytes, and glial cells from NPC1 mice. J. Lipid Res. In press/Published online (2003)
  64. Lin, S., Lu, X., Chang, C.C.Y., and Chang, T.Y. Human Acyl-Coenzyme A: Cholesterol Acyltransferase 2 (hACAT2) expressed in Chinese hamster ovary cells: membrane topology and active site location. Mol. Biol. Cell. In press/Published online (2003)
  65. Sugii, S., Reid, P.C., Ohgami, N., Du, H., and Chang, T.Y. Distinct endosomal compartments in early trafficking of low density lipoprotein-derived cholesterol. J. Biol. Chem. In revision. (2003)
  66. Sakashita, N., Miyazaki, A., Chang, C.C.Y., Chang, T.Y., Kiyota, E., Satoh, M., Komohara, Y., Morganelli, P., Horiuchi, S., and Takeya, M. Acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) is induced in monocyte-derived macrophages: in vivo and in vitro studies. Lab. Invest. In revision. (2003)

 

Invited Reviews in Books, Review Series, and Journals:

  1. Chang, T.Y., Prescott, L.M., and Jones, M.E. Aspartate Transcarbamylase (Streptococcus faecalis) in Methods in Enzymology (Hoffee, P.A. and Jones, M.E., Eds), Academic Press, New York, vol. II, p. 41 (1978)
  2. Chang, T.Y. in The Enzymes, 3rd edition, vol. 16 on Lipid Metabolism (P.D. Boyer, Ed.) Chapter 14 on "Mammalian HMG-CoA Reductase and Its Regulation", Academic Press, 491-521 (1983)
  3. Chang, T.Y. and Doolittle, G.M. in The Enzymes, 3rd edition, vol. 16 on Lipid Metabolism (P.D. Boyer, Ed.) Chapter 15 on "Acyl-Coenzyme A:Cholesterol Acyltransferase", Academic Press, 523-539 (1983)
  4. Chang, T.Y., Chang, C.C.Y., and Cadigan, K.M. Structure of Acyl-Coenzyme A:Cholesterol Acyltransferase and its Potential Relevance to Atherosclerosis. Trends in Cardiovascular Medicine 4, 223 (1994)
  5. Chang, T.Y., Chang, C.C.Y., and Cheng, D. Acyl-Coenzyme A:Cholesterol Acyltransferase. Ann. Rev. Biochem. 66, 613 (1997)
  6. Chang, T.Y., Hasan, M., Chin, J., Chang, C.C.Y., Spillane, D., and Chen, J. Chinese Hamster Ovary Cell Mutants Affecting Cholesterol Metabolism. Current Opinions in Lipidology 8, 65 (1997)
  7. Chang, T.Y. SREBPs, Membrane Lipid Biosynthesis, and Fatty Acids. J. Clinical Invest. 100, 1905 (1997)
  8. Chang, T.Y., Chang, C.C.Y., and Lee, O. in Intracellular Cholesterol Trafficking (Ed. by T.Y. Chang and D. Freeman). Kluwer Press. Chapter on "The Sterol-Specific Regulation of ACAT-1 and SREBPs in Mammalian Cells and in Liver", pp.1-14 (1998)
  9. Chang, T.Y., and Chang, C.C.Y. in Encyclopedia of Molecular Medicine (Edited by T.E.Creighton), Entry on "Acyl-Coenzyme A:Cholesterol Acyltransferase", Wiley & Sons. (2001)
  10. Chang, T.Y., Chang, C.C.Y., Lu, X.H., and Lin, S. Catalysis of ACAT may be completed within the plane of the membrane: a working hypothesis. J. Lipid Research 42, 1933 (2001)
  11. Chang, T.Y., Chang, C.C.Y., Lin, S., Yu, C., Li, B.L., and Miyazaki, A. Roles of acyl-coenzyme A:cholesterol acyltransferase-1 and-2. Current Opin. Lipidol. 12, 289 (2001)
  12. Chang, T.Y., Chang, C.C.Y. ACAT as a Target for Pharmaceutical Intervention of Atherosclerosis. Invited commentary published on the International Atherosclerosis Society website (www.athero.org), August 2002.

 

Books:

1. Intracellular Cholesterol Trafficking (Ed. by T.Y. Chang and D. Freeman). Kluwer Academic Press. (1998)

 

Patents:

Inventor of three patents issued to Dartmouth College:

1. U.S. Patent # 5,484,727 (Issued 01/16/96)

2. U.S. Patent # 5,834,283 (Issued 11/10/98)

3. U.S. Patent # 5,968,749 (Issued 10/19/99)