Expression of Human Single Chain Interleukin 12 Gene in Drosophila Cells

JIN Li^*, QI Xu¹, LAI Bao-Chang¹, SI Lü-Sheng¹, WANG Yi-Li¹
( Central Laboratory of Shanghai No.1 Hospital, Shanghai 200080, China；¹Medical College, Xi'an Jiao Tong University, Xi'an 710061, China )

Abstract    The hscIL-12 gene(no signal sequence), amplified by PCR, was subcloned into an expressing vector pMT/Bip/V5. The recombinant plasmid was transferedinto Drosophila cell line S2. The secreted hscIL-12 fusion protein was identified by Western blot, and the bioactivity of hscIL-12 was investigated by lymphocyte proliferation assay. The results showed hscIL-12 expressed in S2 cells could stimulate lymphocyte proliferation in dose-dependent manner. hscIL-12 has bioactivity identical to natural interleukin 12. These findings suggest that hscIL-12 is promising in theoretical reseach and in clinical use.
Key words    interleukin 12； bioactivity； gene expression

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