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Vol 47 No. 7: 530-538 |
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Down-regulation of microRNA-26b modulates non-small cell lung cancer cells chemoresistance and migration through the association of PTEN |
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Naixin Liang1,†,*,
Xiaoyun Zhou1,
Ming Zhao2,
Dachun Zhao3,
Zhaohui Zhu4,
Shanqing Li1 and
Huaxia Yang5,†
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1Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing 100730, China
2Department of Thoracic Surgery, The General Hospital of the People’s Liberation Army Hospital, Beijing 100853, China
3Department of Pathology, Peking Union Medical College Hospital, Beijing 100730, China
4Department of Rheumatology, Peking Union Medical College Hospital, Beijing 100730, China
5Department of Nuclear Medicine, Peking Union Medical College Hospital, Beijing 100730, China
† These authors contributed equally to this work.
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Abstract To explore the effect of microRNA-26b (miR-26b) in non-small cell lung cancer (NSCLC) cells, we investigated the mRNA levels of miR-26b in 4 NSCLC cell lines and 10 clinical samples from human patients with NSCLC by quantitative reverse transcriptase polymerase chain reaction. It was found that miR-26b was significantly down-regulated in both NSCLC cells and human carcinoma tissues. Synthetic oligonucleotides were used to up-regulate or down-regulate miR-26b in NSCLC cell lines H1299 and A549 cells. Results showed that both down-regulating and up-regulating miR-26b had no effect on cancer cell proliferation in H1299 or A549 cells, whereas miR-26b over-expression increased cancer cell migration and reduced cisplatin chemosensitivity. Phosphatase and tensin homolog (PTEN) was confirmed to be directly bound by miR-26b by dual-luciferase reporter assay, and was down-regulated in miR-26b over-expressing NSCLC cells. Finally, when PTEN was up-regulated in NSCLC cells, it reversed the effects of miR-2b over-expression on NSCLC migration and cisplatin chemosensitivity. In conclusion, our data showed a functional mechanism of miR-26b in regulating NSCLC. It indicates that miR-26b may regulate NSCLC migration and chemosensitivity through the regulation of PTEN.
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Received 2014-12-29
Accepted 2015-2-4
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Funding NSCLC; miR-26b; PTEN; migration; cisplatin
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* Correspondence address Tel/Fax: +86-10-69155001; E-mail: [email protected]
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Browse:1430 |
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