??????
1974.12
1978.4
????????????????
1978.4
1978.8
???????????
1978.9
1982.7
?????????????
1982.8
1985.12
?????????????
1986.1
1989.12
??????????????????
1989.12
1991.6
???????????????
1991.6
1992.6
????????????
1992.7
1994.6
?????????????????????????????????????????????
1994.7
?
?
???????????????????????????
1995.9
1997.3
??????????????????????
1999.7
?
?
???????????????
2000.7
?
?
??????????????????
2001.7
?
?
???863?????????????????????
2001.9
?
?
??????
2002.5
?
?
?????????????????
2002.9
?
?
?????????????????????
????????
1???????????????????????????PPF-1?GDA-1?AAIR????????????????????????????????
??cDNA???????Representational
Difference Analysis–RDA???????????G2????????????????????????????????????????????????????????????????mRNA????????????????????????????????PPF-1?????????????????????????????????????30%????????PPF1???????GFP??????????????????????????????????????????????????????????????????????????????35S????????????????????????????????PPF1?????????????????????????????????????????????????????????????????????????????????????PPF1?+???????????????????????????????PPF1?–????????????????????????????????????PPF1????????????????????????????PPF1????????????????????????????PPF1?????????????????????????????????????????????????????PPF1???????????????????????????????????cDNA??????????????????????????????????????????????????????????????????????????????????????????(?????????1997?2003?Plant
Physiol.?1997?Gene,
1997?MGG?1998????????2001????????2002?The Plant Journal.?2003)
2???????142?????????????fuzless-lintless,
fl?????????????????????????????????????????????????????????????????????????fl????????????????????wildtype?WT???????????????????????????????????10??10DPA,
10 days post anthesis???????????????????fl?????????????PCR-Select
cDNA Subtraction??????????cDNA????????????616????????280??????PCR???????????????????????Array???????????cDNA???????????172?????????????????????????????????????????2002?Biochemical and Biophysical Research
Communications?2002?Nucleic Acids Res.?2003?
3?????????????????????????????????????????MPT-64?Ag85B?ESAT-6?MPT-63?MPT-83??????????????????????????????pJW4303
DNA???????????DNA????Ag85B+MPT-64+ESAT-6?Ag85B+MPT-83+ESAT-6?????????????????100%???????????BCG??????80-100?????????3??????????????????21?????????????????????????????????DNA?????????????75-80%??????????????????????75%-80%????????????0?BCG??????????20%???????????2002????????2003???????????????2003???
3???????????????????????????????70???????????????????????????21.2???????????????PCR????18??????????????????????20????????????????????????????????????16???cDNA???EMBL?GenBank?????????????????X78340?X94352?X96555?X95565?X96609?X92077?X92846?X95566?AF521240?AY218846?AY189968?AY207316?AY189970?AY189969?AY189971?AY189972???GenBank???166??CB350396?CB350561??????????????EST?
????????????2000?????????
1.
*Sheng-Jian Ji, Ying-Chun Lu, Jian-Xun Feng, Gang Wei, Jun Li,
Yong-Hui Shi, Qiang Fu, Di Liu, Jing-Chu Luo and Yu-Xian Zhu. (2003) Isolation
and Analyses of Genes Preferentially Expressed During Early Cotton Fiber
Development by Subtractive PCR and cDNA Array. Nucleic Acids Res. 31: in press.
2.
*????????????????? ????????(2003) ?????????????????????????????????
3.
??????????????????(2003) ???????AaHIT1????????????????????????????19?182-185?
4.
?????????????????(2003) MPT63????????????????????????????19?205-209?
5.
*Dayong Wang, Yunjian Xu, Qing Li, Xuemei Hao, Yanhui Su, Fangzhen
Sun, Yuxian Zhu. (2003) Transgenic expression of a putative calcium transporter
affects the time of arabidopsis flowering. The Plant Journal?33: 285-292
6.
*??????????B????Peter J. Davies??????2003???????????????????????????????????????33?19-26
7.
*????????????????????(2003) ????????DNA????????????????????????35: 71-76?
8.
?????????????????(2003) GDA2??????????????????????????????11: 6-10?
9.
*Yun-Jian Xu, Da-Yong Wang, Yu-Xian Zhu (2002) Expression of the
thylakoid membrane localized PPF1 in transgenic Arabidopsis affects
chloroplast development. Acta Botanica Sinica, 41: 1314-1320?
10.
??????????????????????2002?PPF1??C????????????????????????????????????????18: 594-599?
11.
??????????????2002???????MPT-64??????????????????????????????????18(4):1-4
12.
??????????????2002??????????????????????????????????????????5?24?325-330
13.
*???????????????????2002?????????????????????????????47: 966-971. Cai Hong, Pan
Yi, Li Guoli, Zhuang Yuhui, Zhu Yuxian. (2002) Construction of multivalent DNA
vaccines for Mycobacterium tuberculosis and its immunogenicity. Chinese Science
Bulletin, 47(19): 1589-1593
14.
*Sheng-Jian Ji, Feng Liu, Er-Qiu Li, Yu-Xian Zhu. (2002)
Recombinant scorpion insectotoxin AaIT kills specifically insect cells but not
human cells. Cell research, 12(2): 143-150.
15.
*Shengjian Ji, Yingchun Lu, Jun Li, Gang Wei, Xiujuan Liang,
Yuxian Zhu. (2002) A b-tubulin-like
cDNA expressed specically in elongating cotton fibers induces longitudinal
growth of fission yeast. Biochemical and Biophysical Research Communications.
296 (5): 1245-1250.
16.
*Lu Yingchun, Wei Gang, Zhu Yuxian (2002) Clong whole
cellulose-synthesizing operon (ayacs Operon) from Acetobacter xylinum and
transforming it into cultivated cotton plants. Acta Botanica Sinica. 44:
441-445
17.
*Wang Dayong, Hu Shuang, Li Qing, Cui Keming and Zhu Yuxian
(2002) Photoperiod control of the apical bud and leaf senescence in pumpkin
strain 185. Acta Botanica Sinica. 44: 55-62
18.
*???????Vincent Vachon????????Raynald Laprade? ????2002????????Cry1Aa??à4??????????????????????????????32: 146-152?SuYanhui, Qu Hong, VACHON Vincent, Lu Jingchu, ZHANG
Jie, LAPRADE Raynald and Zhu Yuxian (2002) The toxicity test and
hypothetical model of Bacillus
thuringiensis Cry1Aa helix4. Science In China. 45: 561-567
19. ???????2001?????????????????????????4?5-9?
20.
*????????????????????????2001???????????????cDNA???????????????????46?1022-1026?Xu Huasong, Xu
Yunjian, Gu Xuesong, Hu Tingzhang, Su Yanhui and Zhu Yuxian (2001) Cloning and
analysis of a cDNA encoding acetohydroxy acid isomeroreductase from G2 pea.
Chinese Science Bulletin, 46: 1808-1812. IF 0.511
21.
*Junli Zhou, Ligeng Ma, Suqiao Zhang, Yuxian Zhu, Daye Sun.
(2001) Extracellular calmodulin stimulates light-independent RbcS-GUS
expression in suspension-cultured cells of transgenic tobacco. Plant Cell
Physiol, 42: 1049-1055. IF 2.30
22.
*Guoying Jiang, Yunjian Xu, Xinsheng Zhu, Yanhui Su and Yuxian
Zhu (2001) Prokaryotically expressed Buthus martensii Karsch insect depressant
toxin has insecticidal effects. Toxicon.
39: 469-476. IF 1.366
23.
?????????????????????????2001???PCR????537?????????????????????????????17: 65-67
24.
??? ???????????????????2001????????????????RFLP??PCR??????????????????????????23?175-179
25.
*Huang Jianqiu, Wei Zhiming, An Hailong and Zhu Yuxian (2001)
Agrobacterium tumefaciens-mediated transformation of rice with the spider
insecticidal gene conferring resistance to leaffolder and striped stem borer.
Cell Research, 11: 149-155. IF 2.00
26.
*Yuxian Zhu, James M. Tepperman, Crag Fairchild and Peter H.
Quail (2000) Phytochrome B binds with greater apparent affinity than phychrome
A to the basic helix-loop-helix factor PIF3 in a reaction requiring the PAS
domin of PIF3. Proc. Natl. Acad.
Sci. 97:13419-13424
27.
*????????????????????2000??????cDNA??????????????? ?????45: 1852-1857?Xuede Wang,
Yuxian Zhu, Daofan Ji, Shuli Jiang and Yuyou Li (2000) Cloning of cotton
fiber-related cDNA and its structure variants in four cotton fiber mutants.
Chinese Science Bulletin, 45: 1852-1857
28.
??????????????????? (2000) ???PCR?????cDNA??????????????16: 751-754
29. *????????????????2000??BmKIT4??????????????42?732?735?Tingzhang Hu,
Yunjian Xu, Guoying Jiang and Yuxian Zhu (2000)?Studies on
insect resistance of transgenic tobacco plants carrying the BMK IT4 gene. Acta Botanica Sinica?42?732?735