Yu-Xian
??????
1974.12
1978.4
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1978.4
1978.8
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1978.9
1982.7
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1982.8
1985.12
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1986.1
1989.12
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1989.12
1991.6
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1991.6
1992.6
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1992.7
1994.6
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1994.7
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1995.9
1997.3
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1999.7
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2000.7
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2001.7
? ?
???863?????????????????????
2001.9
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2002.5
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2002.9
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Major
Research Areas:
My laboratory is interested in two major
research areas: large-scale cloning and analyses of genes involved in early
cotton fiber development, and tuberculosis
DNA vaccine development. Using cDNA prepared from 10dpa (days post anthesis)
wild-type cotton fiber as tester and cDNA from a fuzzless-lintless (fl)
mutant as driver, we applied the RDA method to recover 280 independent cDNA
fragments related directly to early cotton fiber development. cDNA microarrays
showed that 172 genes were significantly up-regulated in elongating cotton
fibers as confirmed by in situ hybridization in representative cases.
Twenty-nine cDNAs, including a putative vacuolar (H+)-ATPase
catalytic subunit, a kinesin-like calmodulin binding protein, several
arabinogalactan proteins and key enzymes involved in long chain fatty acid
biosynthesis, accumulated to greater than 50-fold in 10 dpa fiber cells when
compared to that in 0 dpa ovules. Various upstream pathways, such as auxin
signal transduction, the MAP kinase pathway, and profilin- and expansin-induced
cell wall loosening, were also activated during the fast fiber elongation
period.
We have cloned
five genes encoding different secreted antigens Ag85B, MPT-83, MPT-64, MPT-63
and ESAT-6 from Mycobacterium Tuberculosis. We are currently developing
either combined or polyvalent DNA vaccines with different combinations.
Experimental results obtained both from mice and from cows showed that
immunization of combined DNA vaccines expressing 3 or 4 secreted antigens might
produce significant protection against microbial invasion. Our data indicated
that antigen-specific IFN-?levels, rather than antibody levels, might be a
better criteria for protective efficacy assessment.
Besides the above two major areas, the
lab is also involved in cloning and expressional analyses of 8
large gene families (AP2-EREBP?bHLH?C3H-TYPE2(Zn)?E2F-DP?HMG-BOX?NIN-like?PCG?TCP) encoding Arabidopsis transcription factors. We have so far obtained about 250 full-length cDNAs and are
currently working on yeast expression as well as gel mobility shift assays to
study their DNA binding activities. We have done some work on elucidating
molecular mechanisms of plant apical senescence using G2 pea as a model system.
We showed that transgenic Arabidopsis plants carrying a putative chloroplast
membrane-localized calcium channel protein (the PPF1 gene) in sense
orientation flowered much later compared to control plants whereas antisense
expression of this gene rendered the recipients a very rapid life cycle. The
strength of this late-flowering phenotype correlated roughly with the level of PPF1
transcripts and with the calcium storage capacity in the chloroplasts of
different transgenic lines. We suggest that PPF1 is a novel calcium ion carrier
that regulates flowering time of higher plants by changing the Ca2+
storage capacity within chloroplasts.
????????????2000?????????
*Sheng-Jian Ji, Ying-Chun
Lu, Jian-Xun Feng, Gang Wei, Jun Li, Yong-Hui Shi, Qiang Fu, Di Liu, Jing-Chu
Luo and Yu-Xian Zhu. (2003) Isolation and Analyses of Genes Preferentially
Expressed During Early Cotton Fiber Development by Subtractive PCR and cDNA
Array. Nucleic Acids Res. 31: in
press.
*????????????????? ????????(2003) ?????????????????????????????????
??????????????????(2003) ???????AaHIT1????????????????????????????19?182-185?
?????????????????(2003) MPT63????????????????????????????19?205-209?
*Dayong Wang, Yunjian Xu, Qing Li, Xuemei
Hao, Yanhui Su, Fangzhen Sun, Yuxian Zhu. (2003) Transgenic expression of a
putative calcium transporter affects the time of arabidopsis flowering. The
Plant Journal?33: 285-292
*??????????B????Peter J. Davies??????2003???????????????????????????????????????33: 19-26
*????????????????????(2003) ????????DNA????????????????????????35: 71-76?
?????????????????(2003) GDA2??????????????????????????????11: 6-10?
*Yun-Jian Xu, Da-Yong Wang,
Yu-Xian Zhu (2002) Expression of the thylakoid membrane localized PPF1
in transgenic Arabidopsis affects chloroplast development. Acta Botanica
Sinica, 41: 1314-1320?
??????????????????????2002?PPF1??C????????????????????????????????????????18: 594-599?
??????????????2002???????MPT-64??????????????????????????????????18(4):1-4
??????????????2002??????????????????????????????????????????5?24?325-330
*???????????????????2002?????????????????????????????47: 966-971. Cai Hong, Pan
Yi, Li Guoli, Zhuang Yuhui, Zhu Yuxian. (2002) Construction of multivalent DNA
vaccines for Mycobacterium tuberculosis and its immunogenicity. Chinese Science
Bulletin, 47(19): 1589-1593
*Sheng-Jian Ji, Feng Liu, Er-Qiu Li, Yu-Xian Zhu. (2002)
Recombinant scorpion insectotoxin AaIT kills specifically insect cells but not
human cells. Cell research, 12(2): 143-150.
*Shengjian Ji, Yingchun Lu, Jun Li, Gang Wei, Xiujuan Liang, Yuxian
Zhu. (2002) A b-tubulin-like cDNA expressed
specically in elongating cotton fibers induces longitudinal growth of fission
yeast. Biochemical and Biophysical Research Communications. 296 (5): 1245-1250.
*Lu Yingchun, Wei Gang, Zhu Yuxian (2002) Clong whole
cellulose-synthesizing operon (ayacs Operon) from Acetobacter xylinum and
transforming it into cultivated cotton plants. Acta Botanica Sinica. 44:
441-445
*Wang Dayong, Hu Shuang, Li Qing, Cui Keming and Zhu Yuxian (2002)
Photoperiod control of the apical bud and leaf senescence in pumpkin strain
185. Acta Botanica Sinica. 44: 55-62
*???????Vincent Vachon????????Raynald Laprade? ????2002????????Cry1Aa??à4??????????????????????????????32: 146-152?SuYanhui, Qu Hong,
VACHON Vincent, Lu Jingchu, ZHANG Jie, LAPRADE Raynald and Zhu Yuxian
(2002) The toxicity test and hypothetical model of Bacillus thuringiensis Cry1Aa helix4. Science In China. 45: 561-567
???????2001?????????????????????????4?5-9?
*????????????????????????2001???????????????cDNA???????????????????46?1022-1026?Xu Huasong, Xu Yunjian, Gu
Xuesong, Hu Tingzhang, Su Yanhui and Zhu Yuxian (2001) Cloning and analysis of
a cDNA encoding acetohydroxy acid isomeroreductase from G2 pea. Chinese Science
Bulletin, 46: 1808-1812. IF 0.511
*Junli Zhou, Ligeng Ma, Suqiao Zhang, Yuxian Zhu, Daye Sun. (2001)
Extracellular calmodulin stimulates light-independent RbcS-GUS expression in
suspension-cultured cells of transgenic tobacco. Plant Cell Physiol, 42:
1049-1055. IF 2.30
*Guoying Jiang, Yunjian Xu, Xinsheng Zhu, Yanhui Su and Yuxian Zhu
(2001) Prokaryotically expressed Buthus martensii Karsch insect depressant
toxin has insecticidal effects. Toxicon.
39: 469-476. IF 1.366
?????????????????????????2001???PCR????537?????????????????????????????17: 65-67
??? ???????????????????2001????????????????RFLP??PCR??????????????????????????23?175-179
*Huang Jianqiu, Wei Zhiming, An Hailong and Zhu Yuxian (2001)
Agrobacterium tumefaciens-mediated transformation of rice with the spider
insecticidal gene conferring resistance to leaffolder and striped stem borer.
Cell Research, 11: 149-155. IF 2.00
*Yuxian Zhu, James M. Tepperman, Crag Fairchild and Peter H. Quail
(2000) Phytochrome B binds with greater apparent affinity than phychrome A to
the basic helix-loop-helix factor PIF3 in a reaction requiring the PAS domin of
PIF3. Proc. Natl. Acad. Sci.
97:13419-13424
*????????????????????2000??????cDNA??????????????? ?????45: 1852-1857?Xuede Wang, Yuxian Zhu,
Daofan Ji, Shuli Jiang and Yuyou Li (2000) Cloning of cotton fiber-related cDNA
and its structure variants in four cotton fiber mutants. Chinese Science
Bulletin, 45: 1852-1857
??????????????????? (2000) ???PCR?????cDNA??????????????16: 751-754
*????????????????2000??BmKIT4??????????????42?732?735?Tingzhang Hu, Yunjian Xu, Guoying Jiang and Yuxian Zhu (2000)?Studies on insect resistance of transgenic tobacco plants carrying
the BMK IT4 gene. Acta Botanica
Sinica?42?732?735